MCM5

Identifikatori

Alijasi

MCM5

Spoljašnji ID

OMIM: 602696 MGI: 103197 HomoloGene: 4904 GeneCards: MCM5

Genska lokacija (miš)

Hr.	Chromosome 8 (mouse)^[1]

Band	8\|8 C1	Start	75,836,197 bp^[1]
Band	8\|8 C1	Kraj	75,855,067 bp^[1]

Obrazac RNK izražavanja

More reference expression data

Genska ontologija
Molecular function	• nucleotide binding • DNA binding • GO:0004003 DNA helicase activity • DNA replication origin binding • chromatin binding • GO:0001948, GO:0016582 везивање за протеине плазме • hydrolase activity • ATP binding • GO:0008026 helicase activity • single-stranded DNA binding • GO:0043142 single-stranded DNA helicase activity • GO:0043140 3'-5' DNA helicase activity
Cellular component	• MCM complex • мембрана • једро • Нуклеоплазма • цитоплазма • цитосол
Biological process	• G1 phase • репликација ДНК • животни циклус ћелије • DNA duplex unwinding • DNA replication initiation • G1/S transition of mitotic cell cycle • double-strand break repair via break-induced replication • pre-replicative complex assembly involved in nuclear cell cycle DNA replication
Sources:Amigo / QuickGO

Ortolozi

Vrste

Čovek

Miš

Entrez


4174


17218

Ensembl


ENSG00000100297


ENSMUSG00000005410

UniProt


P33992


P49718

RefSeq (mRNA)


NM_006739


NM_008566 NM_001302540

RefSeq (protein)


NP_006730


NP_001289469 NP_032592

Location (UCSC)

n/a

Chr 8: 75.84 – 75.86 Mb

PubMed search

^[2]

^[3]

Wikidata

View/Edit Human

View/Edit Mouse

Faktor licenciranja DNK replikacije MCM5 je protein koji je kod ljudi kodiran MCM5 genom.^[4]^[5]^[6]

Protein kodiran ovim genom je strukturno veoma slian CDC46 proteinu iz S. cerevisiae, proteinu koji učestvuje u inicijaciji DNK replikacije. Ovaj protein je član MCM familije proteina koji vezuju hromatin. On može da formira interakcije sa najmanje dva druga člana ove familije. Ovaj protein je u većoj meri izražen tokom tranzicije iz G0 faze u G1/S fazu ćelijskog ciklusa, i smatra se da aktivno učestvuje u regulaciji ćelijskog ciklusa.^[6]

Vidi još

Održavanje minihromozoma

Interakcije

MCM5 formira interakcije sa ORC2L,^[7] MCM7,^[7]^[8]^[9] STAT1,^[9]^[10] MCM2,^[7]^[9] CDC7,^[7] MCM3^[7]^[9] i ORC6L.^[7]

Reference

^ ^а ^б ^в GRCm38: Ensembl release 89: ENSMUSG00000005410 - Ensembl, May 2017
^ „Human PubMed Reference:”. National Center for Biotechnology Information, U.S. National Library of Medicine.
^ „Mouse PubMed Reference:”. National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Paul R, Hu B, Musahl C, Hameister H, Knippers R (1996). „Coding sequence and chromosome mapping of the human gene (CDC46) for replication protein hCdc46/Mcm5”. Cytogenet Cell Genet. 73 (4): 317—21. PMID 8751386. doi:10.1159/000134366.
^ Dunham I, Shimizu N, Roe BA, Chissoe S, Hunt AR, Collins JE, Bruskiewich R, Beare DM, Clamp M, Smink LJ, Ainscough R, Almeida JP, Babbage A, Bagguley C, Bailey J, Barlow K, Bates KN, Beasley O, Bird CP, Blakey S, Bridgeman AM, Buck D, Burgess J, Burrill WD, O'Brien KP (1999). „The DNA sequence of human chromosome 22”. Nature. 402 (6761): 489—95. PMID 10591208. doi:10.1038/990031.
^ ^а ^б „Entrez Gene: MCM5 MCM5 minichromosome maintenance deficient 5, cell division cycle 46 (S. cerevisiae)”.
^ ^а ^б ^в ^г ^д ^ђ Kneissl, Margot; Pütter Vera; Szalay Aladar A; Grummt Friedrich (2003). „Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells”. J. Mol. Biol. England. 327 (1): 111—28. ISSN 0022-2836. PMID 12614612. doi:10.1016/S0022-2836(03)00079-2.
^ Fujita, M; Kiyono T; Hayashi Y; Ishibashi M (1997). „In vivo interaction of human MCM heterohexameric complexes with chromatin. Possible involvement of ATP”. J. Biol. Chem. UNITED STATES. 272 (16): 10928—35. ISSN 0021-9258. PMID 9099751. doi:10.1074/jbc.272.16.10928.
^ ^а ^б ^в ^г DaFonseca, C J; Shu F; Zhang J J (2001). „Identification of two residues in MCM5 critical for the assembly of MCM complexes and Stat1-mediated transcription activation in response to IFN-gamma”. Proc. Natl. Acad. Sci. U.S.A. United States. 98 (6): 3034—9. ISSN 0027-8424. PMC 30602 . PMID 11248027. doi:10.1073/pnas.061487598.
^ Zhang, J J; Zhao Y; Chait B T; Lathem W W; Ritzi M; Knippers R; Darnell J E (1998). „Ser727-dependent recruitment of MCM5 by Stat1alpha in IFN-gamma-induced transcriptional activation”. EMBO J. ENGLAND. 17 (23): 6963—71. ISSN 0261-4189. PMC 1171044 . PMID 9843502. doi:10.1093/emboj/17.23.6963.

Literatura

Burkhart R, Schulte D, Hu D, Musahl C, Göhring F, Knippers R (1995). „Interactions of human nuclear proteins P1Mcm3 and P1Cdc46.”. Eur. J. Biochem. 228 (2): 431—8. PMID 7705359. doi:10.1111/j.1432-1033.1995.tb20281.x.
Maruyama K, Sugano S (1994). „Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides.”. Gene. 138 (1–2): 171—4. PMID 8125298. doi:10.1016/0378-1119(94)90802-8.
Hu B, Burkhart R, Schulte D, Musahl C, Knippers R (1994). „The P1 family: a new class of nuclear mammalian proteins related to the yeast Mcm replication proteins”. Nucleic Acids Res. 21 (23): 5289—93. PMC 310560 . PMID 8265339. doi:10.1093/nar/21.23.5289-a.
Ishimi Y, Ichinose S, Omori A, Sato K, Kimura H (1996). „Binding of human minichromosome maintenance proteins with histone H3”. J. Biol. Chem. 271 (39): 24115—22. PMID 8798650. doi:10.1074/jbc.271.39.24115.
Fujita M, Kiyono T, Hayashi Y, Ishibashi M (1997). „In vivo interaction of human MCM heterohexameric complexes with chromatin. Possible involvement of ATP”. J. Biol. Chem. 272 (16): 10928—35. PMID 9099751. doi:10.1074/jbc.272.16.10928.
Tsuruga H, Yabuta N, Hashizume K, Ikeda M, Endo Y, Nojima H (1997). „Expression, nuclear localization and interactions of human MCM/P1 proteins”. Biochem. Biophys. Res. Commun. 236 (1): 118—25. PMID 9223437. doi:10.1006/bbrc.1997.6865.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (1997). „Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library”. Gene. 200 (1–2): 149—56. PMID 9373149. doi:10.1016/S0378-1119(97)00411-3.
Zhang JJ, Zhao Y, Chait BT, Lathem WW, Ritzi M, Knippers R, Darnell JE (1999). „Ser727-dependent recruitment of MCM5 by Stat1alpha in IFN-gamma-induced transcriptional activation”. EMBO J. 17 (23): 6963—71. PMC 1171044 . PMID 9843502. doi:10.1093/emboj/17.23.6963.
Ohtani K, Iwanaga R, Nakamura M, Ikeda M, Yabuta N, Tsuruga H, Nojima H (1999). „Cell growth-regulated expression of mammalian MCM5 and MCM6 genes mediated by the transcription factor E2F”. Oncogene. 18 (14): 2299—309. PMID 10327050. doi:10.1038/sj.onc.1202544.
DaFonseca CJ, Shu F, Zhang JJ (2001). „Identification of two residues in MCM5 critical for the assembly of MCM complexes and Stat1-mediated transcription activation in response to IFN-gamma”. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3034—9. PMC 30602 . PMID 11248027. doi:10.1073/pnas.061487598.
Going JJ, Keith WN, Neilson L, Stoeber K, Stuart RC, Williams GH (2002). „Aberrant expression of minichromosome maintenance proteins 2 and 5, and Ki-67 in dysplastic squamous oesophageal epithelium and Barrett's mucosa”. Gut. 50 (3): 373—7. PMC 1773132 . PMID 11839717. doi:10.1136/gut.50.3.373.
Kneissl M, Pütter V, Szalay AA, Grummt F (2003). „Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells”. J. Mol. Biol. 327 (1): 111—28. PMID 12614612. doi:10.1016/S0022-2836(03)00079-2.
Bouwmeester T, Bauch A, Ruffner H, Angrand PO, Bergamini G, Croughton K, Cruciat C, Eberhard D, Gagneur J, Ghidelli S, Hopf C, Huhse B, Mangano R, Michon AM, Schirle M, Schlegl J, Schwab M, Stein MA, Bauer A, Casari G, Drewes G, Gavin AC, Jackson DB, Joberty G, Neubauer G, Rick J, Kuster B, Superti-Furga G (2004). „A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway”. Nat. Cell Biol. 6 (2): 97—105. PMID 14743216. doi:10.1038/ncb1086.
Ramachandran N, Hainsworth E, Bhullar B, Eisenstein S, Rosen B, Lau AY, Walter JC, LaBaer J (2004). „Self-assembling protein microarrays”. Science. 305 (5680): 86—90. PMID 15232106. doi:10.1126/science.1097639.
Yu Z, Feng D, Liang C (2004). „Pairwise interactions of the six human MCM protein subunits”. J. Mol. Biol. 340 (5): 1197—206. PMID 15236977. doi:10.1016/j.jmb.2004.05.024.
Jin J, Smith FD, Stark C, Wells CD, Fawcett JP, Kulkarni S, Metalnikov P, O'Donnell P, Taylor P, Taylor L, Zougman A, Woodgett JR, Langeberg LK, Scott JD, Pawson T (2004). „Proteomic, functional, and domain-based analysis of in vivo 14-3-3 binding proteins involved in cytoskeletal regulation and cellular organization”. Curr. Biol. 14 (16): 1436—50. PMID 15324660. doi:10.1016/j.cub.2004.07.051.

DNK replikacija (Prokariotska, Eukariotska)

Separacija
i inicijacija

Prokariotska (inicijacija)	Prereplikacioni kompleks Cdc6 Helikaza (dnaA, dnaB, T7) · Primaza (dnaG)
Eukariotska (priprema u G1 fazi)	Prereplikacioni kompleks: Kompleks prepoznavanja mesta početka (ORC1, ORC2, ORC3, ORC4, ORC5, ORC6) · Održavanje minihromozoma (MCM2 · MCM3 · MCM4 · MCM5 · MCM6 · MCM7) · CDC6 Faktor licenciranja Autonomna replikaciona sekvenca Protein jednolančanog vezivanja (SSBP1, SSBP2, SSBP3, SSBP4) RNaza H (RNASEH1, RNASEH2A) Helikaza: HFM1 Primaza: PRIM1 · PRIM2
Oba	Početak replikacije/Ori/Replikon Replikaciona viljuška (zaostajući i vodeći lanci) · Okazakijev fragment · Prajmer

Replikacija

Prokariotska (elongacija)	DNK polimeraza III holoenzim (dnaC, dnaE, dnaH, dnaN, dnaQ, dnaT, dnaX, holA, holB, holC, holD, holE) · Replizom · DNK ligaza · DNK stega · Topoizomeraza (DNK giraza) Prokariotska DNK polimeraza: DNK polimeraza I (Klenov fragment)
Eukariotska (sinteza u S fazi)	Replikacioni faktor C (RFC1) · Endonukleaza prepusta (FEN1) · Topoizomeraza · Replikacioni protein A (RPA1) Eukariotska DNK polimeraza: delta (POLD1, POLD2, POLD3, POLD4) DNK stega (PCNA)
Oba	Kretanje: Procesivnost · DNK ligaza

Terminacija

Telomer: Telomeraza (TERT, TERC, DKC1)

B bsyn: dnk (repl, cycl, reco, repr) · tscr (fact, tcrg, nucl, rnat, rept, ptts) · tltn (risu, pttl, nexn) · dnab, rnab/runp · stru (domn, 1°, 2°, 3°, 4°)