Precorrin-8X methylmutase

precorrin-8X methylmutase
Crystal structure of precorrin-8x methyl mutase with bound hydrogenobyrinate. PDB 1i1h[1]
Identifiers
EC no.	5.4.99.61
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
Search PMC articles PubMed articles NCBI proteins

In enzymology, a precorrin-8X methylmutase (EC 5.4.99.61) is an enzyme that catalyzes the chemical reaction

precorrin-8X ${\displaystyle \rightleftharpoons }$ hydrogenobyrinate

Hence, this enzyme has one substrate, precorrin 8X, and one product, hydrogenobyrinate.

This enzyme belongs to the family of isomerases, specifically those intramolecular transferases transferring other groups. The systematic name of this enzyme class is precorrin-8X 11,12-methylmutase. Other names in common use include precorrin isomerase, hydrogenobyrinic acid-binding protein and CobH. This enzyme is part of the biosynthetic pathway to cobalamin (vitamin B₁₂) in aerobic bacteria.

• Cobalamin biosynthesis

As of late 2007, 6 structures have been solved for this class of enzymes, with PDB accession codes 1F2V, 1I1H, 1OU0, 1V9C, 2AFR, and 2AFV.

• Blanche F; Couder, M; Famechon, A; Debussche, L; Cameron, B; Crouzet, J; Blanche, F (1992). "The final step in the biosynthesis of hydrogenobyrinic acid is catalyzed by the cobH gene product with precorrin-8x as the substrate". J. Bacteriol. 174 (3): 1043–9. doi:10.1128/JB.174.3.1043-1049.1992. PMC 206186. PMID 1732194.
• Roth JR, Lawrence JG, Rubenfield M, Kieffer-Higgins S, Church GM (1993). "Characterization of the cobalamin (vitamin B12) biosynthetic genes of Salmonella typhimurium". J. Bacteriol. 175 (11): 3303–16. doi:10.1128/jb.175.11.3303-3316.1993. PMC 204727. PMID 8501034.
• Stolowich NJ, Iida K, Scott AI (1992). "Expression of 9 Salmonella typhimurium enzymes for cobinamide synthesis. Identification of the 11-methyl and 20-methyl transferases of corrin biosynthesis". FEBS Lett. 301 (1): 73–8. doi:10.1016/0014-5793(92)80213-Z. PMID 1451790. S2CID 20198692.
• Thibaut D, Debussche L (1990). "Genetic and sequence analysis of an 8.7-kilobase Pseudomonas denitrificans fragment carrying eight genes involved in transformation of precorrin-2 to cobyrinic acid". J. Bacteriol. 172 (10): 5980–90. doi:10.1128/JB.172.10.5980-5990.1990. PMC 526920. PMID 2211521.