Protein found in humans
GCA |
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![](//upload.wikimedia.org/wikipedia/commons/thumb/7/7f/Protein_GCA_PDB_1f4o.png/250px-Protein_GCA_PDB_1f4o.png) |
Available structures |
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PDB | Ortholog search: PDBe RCSB |
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List of PDB id codes |
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1F4O, 1F4Q, 1K94, 1K95 |
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Identifiers |
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Aliases | GCA, GCL, grancalcin |
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External IDs | OMIM: 607030; MGI: 1918521; HomoloGene: 22702; GeneCards: GCA; OMA:GCA - orthologs |
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Gene location (Human) |
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![Chromosome 2 (human)](//upload.wikimedia.org/wikipedia/commons/thumb/a/aa/Ideogram_human_chromosome_2.svg/300px-Ideogram_human_chromosome_2.svg.png) | Chr. | Chromosome 2 (human)[1] |
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| Band | 2q24.2 | Start | 162,318,840 bp[1] |
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End | 162,371,595 bp[1] |
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Gene location (Mouse) |
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![Chromosome 2 (mouse)](//upload.wikimedia.org/wikipedia/commons/thumb/b/bb/Ideogram_house_mouse_chromosome_2.svg/260px-Ideogram_house_mouse_chromosome_2.svg.png) | Chr. | Chromosome 2 (mouse)[2] |
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| Band | 2|2 C1.3 | Start | 62,494,629 bp[2] |
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End | 62,524,453 bp[2] |
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RNA expression pattern |
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Bgee | Human | Mouse (ortholog) |
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Top expressed in | - monocyte
- blood
- bone marrow
- bone marrow cells
- trabecular bone
- granulocyte
- spleen
- germinal epithelium
- C1 segment
- right lung
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| Top expressed in | - granulocyte
- lumbar spinal ganglion
- morula
- pineal gland
- primary motor cortex
- olfactory epithelium
- seminal vesicula
- sciatic nerve
- ventricular zone
- retinal pigment epithelium
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| More reference expression data |
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BioGPS | ![](//upload.wikimedia.org/wikipedia/commons/thumb/2/2d/PBB_GE_GCA_203765_at_fs.png/250px-PBB_GE_GCA_203765_at_fs.png) | More reference expression data |
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Gene ontology |
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Molecular function | - protein binding
- protein homodimerization activity
- metal ion binding
- calcium-dependent cysteine-type endopeptidase activity
- protein heterodimerization activity
- calcium ion binding
| Cellular component | - plasma membrane
- extracellular exosome
- membrane
- extracellular region
- cytosol
- azurophil granule lumen
- cytoplasm
| Biological process | - membrane fusion
- proteolysis
- neutrophil degranulation
| Sources:Amigo / QuickGO |
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Orthologs |
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Species | Human | Mouse |
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Entrez | | |
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Ensembl | | |
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UniProt | | |
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RefSeq (mRNA) | NM_012198 NM_001330265 NM_001330266 NM_001330267 NM_001330268
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NM_001330270 NM_001330271 |
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RefSeq (protein) | NP_001317194 NP_001317195 NP_001317196 NP_001317197 NP_001317199
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NP_001317200 NP_036330 |
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Location (UCSC) | Chr 2: 162.32 – 162.37 Mb | Chr 2: 62.49 – 62.52 Mb |
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PubMed search | [3] | [4] |
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Wikidata |
View/Edit Human | View/Edit Mouse |
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Grancalcin is a protein that in humans is encoded by the GCA gene.[5][6][7][8]
This gene product, grancalcin, is a calcium-binding protein abundant in neutrophils and macrophages. It belongs to the penta-EF-hand subfamily of proteins which includes sorcin, calpain, and ALG-2. Grancalcin localization is dependent upon calcium and magnesium. In the absence of divalent cation, grancalcin localizes to the cytosolic fraction; with magnesium alone, it partitions with the granule fraction; and in the presence of magnesium and calcium, it associates with both the granule and membrane fractions, suggesting a role for grancalcin in granule-membrane fusion and degranulation.[8]
Interactions
GCA (gene) has been shown to interact with SRI.[7]
References
- ^ a b c GRCh38: Ensembl release 89: ENSG00000115271 – Ensembl, May 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000026893 – Ensembl, May 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ Boyhan A, Casimir CM, French JK, Teahan CG, Segal AW (Mar 1992). "Molecular cloning and characterization of grancalcin, a novel EF-hand calcium-binding protein abundant in neutrophils and monocytes". J Biol Chem. 267 (5): 2928–33. doi:10.1016/S0021-9258(19)50675-5. PMID 1737748.
- ^ Teahan CG, Totty NF, Segal AW (Oct 1992). "Isolation and characterization of grancalcin, a novel 28 kDa EF-hand calcium-binding protein from human neutrophils". Biochem J. 286. ( Pt 2) (2): 549–54. doi:10.1042/bj2860549. PMC 1132932. PMID 1530588.
- ^ a b Hansen C, Tarabykina S, la Cour JM, Lollike K, Berchtold MW (Jun 2003). "The PEF family proteins sorcin and grancalcin interact in vivo and in vitro". FEBS Lett. 545 (2–3): 151–4. doi:10.1016/S0014-5793(03)00518-0. PMID 12804766. S2CID 42572647.
- ^ a b "Entrez Gene: GCA grancalcin, EF-hand calcium binding protein".
Further reading
- Lim J, Hao T, Shaw C, et al. (2006). "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration". Cell. 125 (4): 801–14. doi:10.1016/j.cell.2006.03.032. PMID 16713569. S2CID 13709685.
- Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.
- Hillier LW, Graves TA, Fulton RS, et al. (2005). "Generation and annotation of the DNA sequences of human chromosomes 2 and 4". Nature. 434 (7034): 724–31. Bibcode:2005Natur.434..724H. doi:10.1038/nature03466. PMID 15815621.
- Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
- Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
- Jia J, Borregaard N, Lollike K, Cygler M (2002). "Structure of Ca(2+)-loaded human grancalcin". Acta Crystallogr. D. 57 (Pt 12): 1843–9. doi:10.1107/S0907444901016511. PMID 11717497.
- Lollike K, Johnsen AH, Durussel I, et al. (2001). "Biochemical characterization of the penta-EF-hand protein grancalcin and identification of L-plastin as a binding partner". J. Biol. Chem. 276 (21): 17762–9. doi:10.1074/jbc.M100965200. PMID 11279160.
- Jia J, Han Q, Borregaard N, et al. (2000). "Crystal structure of human grancalcin, a member of the penta-EF-hand protein family". J. Mol. Biol. 300 (5): 1271–81. doi:10.1006/jmbi.2000.3925. PMID 10903868.
- Maki M, Narayana SV, Hitomi K (1998). "A growing family of the Ca2+-binding proteins with five EF-hand motifs". Biochem. J. 328. ( Pt 2) (Pt 2): 718–20. PMC 1218977. PMID 9441591.
PDB gallery
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1f4o: CRYSTAL STRUCTURE OF GRANCALCIN WITH BOUND CALCIUM -
1f4q: CRYSTAL STRUCTURE OF APO GRANCALCIN -
1k94: Crystal structure of des(1-52)grancalcin with bound calcium -
1k95: Crystal structure of des(1-52)grancalcin with bound calcium |
![Stub icon](//upload.wikimedia.org/wikipedia/commons/thumb/e/e2/DNA_stub.svg/40px-DNA_stub.svg.png) | This article on a gene on human chromosome 2 is a stub. You can help Wikipedia by expanding it. |